Cardiac Electrophysiology Overview and Practice Questions

Cardiac Electrophysiology: Overview and Practice Questions

by | Updated: Jan 28, 2023

Cardiac electrophysiology refers to the electrical activity and functionality of the heart. It encompasses the study of both normal and abnormal cardiac findings.

Abnormal cardiac electrophysiology can lead to arrhythmias, which are irregular heart rhythms that can be dangerous.

In this guide, we will provide an overview of cardiac electrophysiology, including how it works and what can go wrong. We included helpful practice questions on this topic as well.

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What is Cardiac Electrophysiology?

Cardiac electrophysiology is the study of the electrical activity of the heart and cardiac system. Cardiac cells generate electrical impulses across the cell membrane creating a polarized state.

The monitoring and observation of cardiac electrical impulses allows physicians to diagnose and treat patients with irregularities of the heart.

Cardiac Electrophysiology Practice Questions:

1. Where is the sinoatrial (SA) node?
It is at the right atria next to superior vena cava entrance.

2. Where is the atrioventricular (AV) node?
It is at the floor of the right atria between the atria and ventricle.

3. Where is the Bundle of His?
It is at the interventricular septum.

4. Where are the Purkinje fibers?
They are innervating throughout the ventricles from the apex towards atrioventricular valves.

5. What is the function of the SA node?
It is the master pacemaker.

6. What is the function of the AV node?
To relay SA node impulse to ventricles.

7. What is the function of the Bundle of His?
It propagates electrical impulses to ventricular apex.

8. What is the function of the Purkinje fibers?
It propagates cardiac action potential throughout ventricles.

9. What are the components of the cardiac electrical conduction system?
Sinoatrial node, Atrioventricular node, Bundles of His, and Purkinje fibers.

10. How are the components of the cardiac electrical conduction system connected?
They are all electrically coupled so the fastest working impulse rate wins.

11. What happens if the SA node is damaged?
Other things can use their pacemaker activity to kick in (even though it’s slower).

12. What are the 3 conduction pathways between the SAN and AVN?
Posterior tract, middle tract (faster than posterior), and anterior tract.

13. What is interesting about the anterior tract between the SAN and AVN?
It bifurcates to Bachmann’s bundle.

14. What is the function of Bachmann’s bundle?
It carries SAN impulse to the left atrium.

15. Is conduction velocity of electrical impulses in the heart equal?

16. What is the slowest stop in the heart?
From the AV node to Bundle of His.

17. What does sympathetic arousal do to heart?
Faster heart rate. Increased funny current, increased Ca2+ current, cAMP increases,
PKA increases and HCN4 stimulated.

18. What does parasympathetic arousal do to heart?
Slower heart rate, increased K+ current, decreased Ca2+ current, decreased funny current,
cAMP decreases, PKA decreases, and HCN4 inhibited.

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19. What is the point of a refractory period?
It keeps action potential from going backwards.

20. What factor affects the width of the QRS complex?
It depends on the velocity of depolarization of the ventricle.

21. In pacemaker cells of the SA node, the action potential is due to the opening of what channels?
The opening of long-lasting (L) Ca2+ channels.

22. Pacemaker potential in pacemaker cells of the SA node is due to?
Decreasing outflow of potassium ions and the opening of transient (T) Ca2+ channels.

23. What cardiac sound does the QRS interval contain?
S1 sound.

24. What ECG segments represents the conduction of an action potential through AV-node and AV-bundle?
P-Q segment and P-R segment.

25. What wave represents atrial repolarization?
Beginning of Q wave to the peak of R wave.

26. What ECG segment represents the completely depolarized ventricles?
S-T segment.

27. What effect on the ventricles can be seen on an ECG?
One or more P waves may occur without initiating a QRS complex. The atria can depolarize regularly, while the ventricles contract at much slower pace.

28. How do you look for heart rate on an ECG?
Beginning of one P wave to the beginning of the next P wave or from peak of one R wave, to the peak of the next R wave.

29. Tachycardia can be seen on ECG as?
A closer distance between two P waves or between two T waves.

30. Bradycardia can be seen on ECG as?
A further distance between two P waves or between two T waves.

31. What is the area that connects cardiac cells?
Intercalated disks.

32. What is the area between cardiac cells where permeability is the greatest which allows the electrical signal to pass quickly between the cells?
Gap junctions.

33. What allows an electrical current to easily flow into individual cardiac cells?
Transverse tubules.

34. What substance enters the cell when it is electrically activated?

35. What is the resting membrane potential value?
-90 mV.

36. What accounts for the resting membrane potential being a negative value?
The cell is highly permeable to potassium, so it leaves the cell to enter a lower concentration gradient.

37. What can result from having a lower than normal concentration gradient?
Decreased electrical conduction velocity.

38. Do the SA/AV node have higher or lower threshold potentials than the rest of the cardiac cells?
Lower (-30 to -40 mV vs -60 to -70 mV).

39. What is happening during stage 0 of the cardiac cell action potential?
Depolarization, the fast-acting sodium channels are open.

40. What is happening during stage 1 of the cardiac cell action potential?
Initial repolarization, the fast-acting potassium channels are open and sodium channels are closed.

41. What is happening during stage 2 of the cardiac cell action potential?
A leveling off at 0 charge, slow acting calcium channels are open and potassium channels are still open.

42. What is happening during stage 3 of the cardiac cell action potential?
Late repolarization, calcium channels close but sodium channels still open.

43. What is happening during stage 4 of the cardiac cell action potential?
Resting state, all channels are closed, only the sodium/potassium pump is working.

44. What is happening during the p wave of the ECG complex?
Depolarization of the atria beginning with SA node.

45. What is happening during the QRS complex of the ECG complex?
Ventricular depolarization which it also covers up atrial repolarization.

46. What is happening during the T wave of the ECG complex?
Ventricular repolarization.

47. What is happening during the U wave of the ECG complex?
Purkinje fiber repolarization.

48. What is happening during the P-R segment of the ECG complex?
The ventricles are filling with blood from the atria.

49. What happens at the J-point of the ECG complex?
When ventricular polarization ends.

50. What happens during the S-T segment of the ECG complex?
The time between the end of ventricular depolarization and start of repolarization.

51. What is needed for the heart pump to meet the needs of the body?
Contraction must be of enough force to eject appropriate volume of blood at adequate pressures. Cardiac muscle cells must contract regularly, at an appropriate rate, and synchronously. Valves must have enough diameter when open. Valves must close fully. And an
adequate filling must occur during diastole.

52. What triggers an action potential?
Depolarization of the plasma membrane and a shift in the membrane potential to a less negative value.

53. What is the threshold potential?
It is the critical level to which a membrane potential must be depolarized to initiate an action potential.

54. During an action potential, which ions temporarily dominate?
Na+ and Ca+

55. What are the pacemakers of the heart?
SA node, AV node, and Purkinje fibers.

56. What is a pacemaker?
It is the spontaneous time-dependent depolarization of the cell membrane that leads to an action potential.

57. What is the sinoatrial node?
It is in the right atrium. Fastest pacemaker and primary site of origin of electrical signal which sets the heart rate in normal conditions.

58. What is the atrioventricular node?
It is located just above the atrioventricular ring. It does not set the heart rate in normal conditions and only when SA node fails.

59. What are Purkinje fibers?
They originate in the AV node. They conduct an electrical signal from atria to right and left ventricles. Tertiary pacemakers – only become the primary pacemaker in case SA and AV nodes fail.

60. What is the normal source of the initiation in an electrical cycle?
Sinoatrial Node (SA Node).

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61. What causes the depolarization and repolarization of the heart?
Once an electrical cell generates an electrical impulse, this electrical impulse causes the ions to cross the cell membrane and causes the action potential, also called depolarization.
Repolarization is the return of the ions to their previous resting state, which corresponds with the relaxation of the myocardial muscle.

62. What are the normal values of the electrolytes inside the cardiac cell?
Sodium (Na) = 5mEq/L, Potassium (K) = 145 mEq/L, and Calcium (Ca) = 1 mEq/L.

63. What are the normal values of the electrolytes outside the cardiac cell?
Sodium (Na) = 145 mEq/L, Potassium (K) = 5 mEq/L, and Calcium (Ca) = 4 mEq/L.

64. What part is the origination of a nodal rhythm?
AV Node

65. What are some of the causes of a nodal rhythm?
Hypoxemia, caffeine, nicotine, electrolyte imbalance, and some drugs.

66. What is cardiac reserve?
The difference between resting cardiac output and maximum cardiac output.

67. What happens during a myocardial infarction?
Myocardium death due to ischemia.

68. How do we treat a myocardial infarction?
Angioplasty, coronary bypass surgery, and stent.

69. What is CHF and its causes?
Congestive Heart Failure (CHF) occurs when one ventricle is not as efficient as the other ventricle.

70. What is pulmonary edema?
It is the fluid accumulation in the tissue and air spaces of the lungs, leading to impaired gas exchange and respiratory failure.

71. What is systemic edema?
An abnormal accumulation of fluid in the interstitium, located beneath the skin and in the cavities of the body which can cause severe pain. Clinically, edema manifests as swelling.

72. What are the characteristics of a primary cardiac cell?
Automaticity, excitability, conductivity, and contractility.

73. What is a Sinus Rhythm?
Normal Heart Rate.

74. What is the Lubb sound?
The closing of AV valves during ventricular diastole.

75. What is the Dubb sound?
The closing of Semilunar Valves during ventricular systole.

76. What is systole?
The Contraction that Increases pressure and decreases volume.

77. What is diastole?
It is relaxation that decreases pressure and increases volume.

78. What is the cardiac cycle?
It is one complete contraction and relaxation of the heart.

79. What is an arrhythmia?
Abnormal heart rhythm.

80. What are the two refractory periods?
(1) Absolute – the time in which the cells cannot respond to a stimulus. Phases 0, 1, 2, and half of 3 represent the absolute refractory period. (2) Relative – the time in which repolarization is almost complete and a strong stimulus may cause depolarization. The second half of phase 3 represents the relative refractory period.

Final Thoughts

Cardiac electrophysiology is a complex topic, but understanding the basics can be helpful in diagnosing and treating cardiac conditions. We hope this guide has been helpful in providing some background information on the topic.

We have a similar article on the cardiovascular system that I think you’ll find helpful. Thanks for reading and, as always, breathe easy, my friend.

Written by:

John Landry, BS, RRT

John Landry is a registered respiratory therapist from Memphis, TN, and has a bachelor's degree in kinesiology. He enjoys using evidence-based research to help others breathe easier and live a healthier life.


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  • “The Current State of Cardiac Electrophysiology.” National Center for Biotechnology Information, 2015,
  • Holzem, Katherine. “Human Cardiac Systems Electrophysiology and Arrhythmogenesis: Iteration of Experiment and Computation.” PubMed, Nov. 2014,

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